Tools to process images and FCS data for FCS-calibrated imaging and high-throughput FCS. The package contains tools to batch fit auto-correlation curves to mathematical models, compute an FCS-calibration curve, and apply the FCS-calibration to images. Refer to the Wiki for more information. For the most recent binary installers to FCSFitM (setup_FCSFitM) and FCSCalibration (setup_FCSCalibration) download the files from the binaries link. The source code can be found following the source code link.
The Fluctuation Analyzer is a software tool for the interactive as well as automated processing of fluorescence auto- and cross-correlation spectroscopy (FCS/FCCS) data. It can read raw data, i.e., one-or two-channel photon streams, from various commercial suppliers of FCS/FCCS data acquisition equipment, organize such data in processing sessions by file-based management, calculate temporal auto- and cross-correlation functions, correct for photobleaching, cross-talk, and background signal and fit the data with appropriate model functions before saving the results. Version 15.02.23 can be downloaded from here. For more recent versions follow the Website link.
The CellCognition framework is designed to combine object detection and supervised machine learning for classification of morphologies with time-resolved analysis by single-cell tracking. This enables measurements of progression through morphology stages and kinetic readouts at the single-cell level.
The software was designed to run on the three major platforms (Windows, Mac OSX, Linux) at high speed with high convenience. We therefore combined C++ image processing based on VIGRA with a Python-based workflow engine and a Qt-based graphical user-interface.