Systems Biology of Mitosis

In humans we do not have a comprehensive understanding of the cell division process, one of the most fundamental functions of life. To address this, we take a comprehensive approach to cell division at the genomic, proteomic, and cellular systems level. After identifying most human genes required for mitosis by high throughput microscopy (www.mitocheck.org), we have developed imaging based in vivo proteomics technology to systematically characterize the abundance, subcellular distribution, and interactions of all mitotic proteins in live dividing cells. The imaging technologies for cellular systems biology are generic and we constantly disseminate them to the scientific community by open source software, practical courses, as well as by giving open access to them in EMBL’s Advanced Light Microscopy Facility, and eventually commercializing them with industrial partners.