Understanding how the genome is organized and compartmentalized inside the cell is fundamental to understand how it functions, can be duplicated and passed on to the next generation. Our research on structure and biogenesis of the nucleus currently focusses on the biophysical characterization of the native structure and dynamics of chromosomes, the structure and assembly of the nuclear pore complexes, and the targeting mechanism of membrane proteins to the nuclear envelope. These studies are carried out in cultured mammalian cell lines and also serve to develop new imaging technologies with higher resolution and more direct mechanistic insight into protein function.
Traces chromatin organization in mammalian nuclei with super-resolution microscopy.
Seeks to unravel the structure and dynamics of interphase NPC biogenesis by integrating live cell imaging with super-resolution microscopy.
Chases epigenetic marks along the path of DNA with super-resolution microscopy.
Inspects the 3D path of DNA through whole chromosomes with super-resolution microscopy.
Investigates the molecular mechanism of NPC assembly with high-resolution fluorescence, single-molecule and correlative techniques.
Dissects the molecular mechanism of membrane deformation during NPC assembly.