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Our group is an international interdisciplinary team drawing its members from biology, physics, chemistry, computer science, and engineering. The overarching theme of the lab is to understand the molecular mechanism of the nuclear division cycle in a comprehensive manner in the physiological context of the intact living cell. To achieve this we develop and use a braod range of fluorescence-based imaging technologies to assay the functions of the involved molecular machinery non-invasively, automate imaging to address all its molecular components and computationally process image data to extract biochemical and biophysical parameters in order to generate mechanistic understanding and predictive models. Our biological questions are currently focused on three areas.